In order for the embryo to attach to the uterine wall during IVF treatment, it must emerge from the protective membrane surrounding the embryo (zona pelllucida) and sprout. This budding occurs until the blastocyst forms on the 5th or 6th day of embryonic development. In order for it to hold in the uterus, it must emerge completely from this membrane. However, sometimes pregnancy cannot occur even if high-quality embryos are obtained during IVF treatment. One of the reasons for this is that this membrane protecting the embryo cannot rupture and the embryo cannot attach to the uterus. For this reason, membrane thinning or an opening process, i.e. assisted hatching (membrane thinning - embryo shaving) is applied to embryos for 2, 3, 4 days before transfer. The medications used in IVF treatment may thicken this membrane surrounding the embryo, or the culture medium developed inside the embryo may harden the membrane. For this reason, during IVF treatment, especially with frozen and thawed embryos, the membrane surrounding the embryo may need to be thinned or opened.
How is assisted hatching applied?
By using a special chemical solution, the membrane surrounding the embryo is thinned out in areas or opened completely.Mechanically, an opening is created in the embryo membrane with the help of a glass microneedle.In the laser method, the embryo membrane is thinned out or an opening is created.
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